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cdk5 1h3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cdk5 1h3
    Chronic palmitate exposure during differentiation disrupts both GSK3β and <t>CDK5</t> signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test
    Cdk5 1h3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1h3/CDK5+Mouse+mAb/pmc12657545-19-0-5
    Average 92 stars, based on 18 article reviews
    cdk5 1h3 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Chronic Fatty Acid Exposure Disrupts SH-SY5Y and Neuronal Differentiation and Is a Potential Link Between Type-2 Diabetes and Alzheimer’s Disease"

    Article Title: Chronic Fatty Acid Exposure Disrupts SH-SY5Y and Neuronal Differentiation and Is a Potential Link Between Type-2 Diabetes and Alzheimer’s Disease

    Journal: Molecular Neurobiology

    doi: 10.1007/s12035-025-05367-6

    Chronic palmitate exposure during differentiation disrupts both GSK3β and CDK5 signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test
    Figure Legend Snippet: Chronic palmitate exposure during differentiation disrupts both GSK3β and CDK5 signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test

    Techniques Used: Western Blot, Control, Expressing

    Related Articles

    other:

    Article Title: Amino acid sequence conservation of the algesic MBP fragment is required for its interaction with CDK5 and function in pain
    Article Snippet: The following antibodies were used: rabbit polyclonal anti-mCherry (Abcam, Cat. No. ab183628); mouse monoclonal anti-CDK5 clone 1H3 (Cell Signaling Technologies, Cat. No. 12134); rabbit polyclonal CDK5 (Bioss Antibody, Cat. No. bs-0559R); rabbit polyclonal anti-laminin antibody (Sigma, Cat. No. L9393), goat polyclonal anti-IL-6 (R&D Systems, Cat. No. AF506, rabbit polyclonal ATF3 (Santa Cruz Biotechnology, Cat. No. SC-188, rabbit polyclonal anti-degraded MBP (Millipore, Cat. No. AB5864) and mouse monoclonal MBP antibodies (Biolegend, Cat. No. 808401).

    Article Title: Amino acid sequence conservation of the algesic fragment of myelin basic protein is required for its interaction with CDK5 and function in pain.
    Article Snippet: The following antibodies were used: rabbit polyclonal antimCherry (Abcam, Burlingame, CA, USA, Cat. No. ab 183628); mouse monoclonal anti-CDK5 clone 1H3 (Cell Signaling Technologies, Danvers, MA, USA, Cat. No. 12134); rabbit polyclonal CDK5 (Bioss Antibody, Woburn, MA, USA, Cat. No. bs-0559R); rabbit polyclonal antilaminin antibody (Sigma-Aldrich, St. Louis, MO, USA, Cat. No. L9393), goat polyclonal anti-IL-6 (R&D Systems, Minneapolis, MN, USA, Cat. No. AF506, rabbit 3496 The FEBS Journal 285 (2018) 3485–3502 a 2018 Federation of European Biochemical Societies nloaded from https://febs.onlinelibrary.w iley.com /doi/10.1111/febs.14623 by C A PE S, W iley O nline L ibrary on [21/01/2024].



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    Image Search Results


    Chronic palmitate exposure during differentiation disrupts both GSK3β and CDK5 signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test

    Journal: Molecular Neurobiology

    Article Title: Chronic Fatty Acid Exposure Disrupts SH-SY5Y and Neuronal Differentiation and Is a Potential Link Between Type-2 Diabetes and Alzheimer’s Disease

    doi: 10.1007/s12035-025-05367-6

    Figure Lengend Snippet: Chronic palmitate exposure during differentiation disrupts both GSK3β and CDK5 signalling in SH-SY5Y cells. A – C Western blotting quantification of GSK3β and GSK3β pS9 in D10 SH-SY5Y cells differentiated in the presence of 20 µM BSA-conjugated palmitic or oleic acid, or an equivalent volume of vehicle. All protein levels are normalised to a GAPDH loading control and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. *** p < 0.001, **** p < 0.0001, 1-way ANOVA with Bonferroni’s post-hoc test. A Total GSK3β. B GSK3β phosphorylated at Ser-9 (pS9 GSK3β), normalised to GSK3β levels. C Representative Western blots. D , E Time course of CDK5 and p35 expressing during differentiation of SH-SY5Y cells under chronic oleate, palmitate or equivalent vehicle exposure. All protein levels are normalised to a GAPDH loading control apart from in C and normalised within each repeat. All values are the mean of four independent repeats, ± SEM. D , E Representative Western blot is shown below each graph. * p < 0.05, *** p < 0.001, 2-way ANOVA with Bonferroni’s post-hoc test

    Article Snippet: CDK5 (1H3) , Mouse , Cell Signaling technologies , 12134 , 1:500.

    Techniques: Western Blot, Control, Expressing